E Tests

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Verotoxogenic E.coli see Haemorrhagic uraemic syndrome

 

Ear infections (otitis media, otitis externa)

 

  • Test Name: Ear infections (otitis media, otitis externa)
  • Laboratory: Microbiology
  • Specimen Type: Swab
  • Specimen Container: Amies swab
  • Specimen Volume: N/A
  • Specimen Requirements: Refrigerate if delivery to the laboratory is delayed
  • Other details:N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: 2-4 days
  • Phone alert limits: Beta Haemolytic Streptococcus Group A isolates, Beta Haemolytic Streptococcus Group B isolates from maternity, post natal and neonatal patients, new MRSA and VRE isolates from inpatients are all phoned to the requesting clinician
  • Preanalytical stability at room temp:N/A

 

Ebstein Barr Virus (EBV) Serology

 

  • Test Name: Ebstein Barr Virus (EBV)
  • Laboratory: Serology
  • Specimen Type: Clotted Blood Specimen
  • Specimen Container: Yellow, white (paediatric).
  • Specimen Volume: 4ml clotted blood (1.5ml paediatric).
  • Specimen Requirements: N/A
  • Other details: N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: 1 day.
  • Phone alert limits:N/A
  • Preanalytical stability at room temp: N/A

 

Ebstein Barr Virus (EBV) PCR

  • Test Name: Ebstein Barr Virus (EBV) PCR
  • Laboratory: Serology External
  • Specimen Type: EDTA Blood Specimen
  • Specimen Container: Purple, red (paediatric).
  • Specimen Volume: 2.5ml clotted blood (1.5ml paediatric).
  • Specimen Requirements: Refrigerate if delivery to the laboratory is delayed.
  • Other details: N/A
  • Frequency of analysis/ assay: Referred to external test site.
  • Turnaround Time: 7-10 days.
  • Phone alert limits: First positive phoned to the requesting clinician.
  • Preanalytical stability at room temp: To be received in Serology Laboratory on day of collection - requires freezing at -20C.

eGFR -Estimated Glomerular Filtration Rate

  • Test Name: eGFR
  • Laboratory: Biochemistry
  • Specimen Type: Serum/Plasma
  • Specimen Container: Yellow (Green for UHW in house samples only)
  • Specimen Volume: 4mls
  • Specimen Requirements: eGFR are calculated on all creatinine results for patients >18yrs age
  • Other details: eGFR is an estimate only. Creatinine must be stable (may be unreliable in acutely ill patients). Not valid in pregnancy, children or at extremes of body type. Multiply by 1.21 for Black race.
  • Frequency of analysis/assay: Daily
  • Turnaround Time: Routine-2 working day. Urgent-< 3hrs
  • Phone Alerts: N/A

  • Preanalytical stability at room temp: N/A

     

    Electrolytes - Serum (Sodium and Potassium)

  • Test Name: Electrolytes - Serum (Sodium and Potassium)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements: Overnight unspun speciemens are unsuitable for potassium.
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: Routine - 2 working day Urgent <3 hours
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Electrolytes - Urinary (Sodium and Potassium)

 

  • Test Name: Electrolytes - Urinary (Sodium and Potassium)
  • Laboratory: Biochemistry
  • Specimen Type: Random urine or 24 hour urine
  • Specimen Container: Sarstedt Urine Z bottle (yellow cap)  or 24 hour urine container.
  • Specimen Volume: 3.2 mls or complete collection
  • Specimen Requirements: Boric acid conatiner (red top) unsuitable.
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: 2 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Electron Microscopy ( Faeces & Fluids )

 

  • Test Name: Electron Microscopy ( Faeces & Fluids )
  • Laboratory: Serology External
  • Specimen Type: Faeces/Fluids
  • Specimen Container: Sterile universal container.
  • Specimen Volume: 1-2g (spoonful). At least 1ml of fluid.
  • Specimen Requirements: N/A
  • Other details: N/A
  • Frequency of analysis/ assay: Referred to external test site
  • Turnaround Time: 7-10 days
  • Phone alert limits:N/A
  • Preanalytical stability at room temp: N/A

 

Electrophoresis (Albumin, alpha1, alpha 2, beta and
gamma globulin)

 

  • Test Name: Electrophoresis (Albumin, alpha1, alpha 2, beta and gamma globulin)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements: Plasma specimen is unsuitable
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: 5 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Endocarditis see also Blood Culture

 

  • Test Name: Endocarditis see also Blood Culture
  • Laboratory: Microbiology
  • Specimen Type: Blood Culture, Valve tissue
  • Specimen Container: Blood culture bottle.
  • Specimen Volume: Blood Culture -5-10ml (1-4ml paediatric) /As large a tissue as possible should be sent
  • Specimen Requirements: In suspected endocarditis, three sets of blood cultures should be taken from separate venepuncture sites.
  • Other details: N/A
  • Frequency of analysis/ assay: N/A
  • Turnaround Time: Cultured for 7 days, Identification and susceptibility within 2 days of growth.
  • Phone alert limits:N/A
  • Preanalytical stability at room temp: N/A

 

Enteric Fever see Blood Cultures

 

Enterobius vermicularis (threadworm/pinworm)

  • Test Name: Enterobius vermicularis (threadworm/pinworm)
  • Laboratory: Microbiology
  • Specimen Type: Sellotape slide
  • Specimen Container: Glass slide placed in slide holder for transport
  • Specimen Volume: Recommended to take up to 5 specimens
  • Specimen Requirements: See sellotape slide in ova and parasite section
  • Other details: Glass slide is taken in the morning on rising, before defecation or bathing.
  • Frequency of analysis/ assay:As required
  • Turnaround Time: 1-3 days
  • Phone alert limits:Positives phoned to requesting clinician.
  • Preanalytical stability at room temp:N/A

 

Enterovirus PCR

  • Test Name: Enterovirus PCR (see also meningitis viral)
  • Laboratory: Serology External
  • Specimen Type: Viral (pink topped) swab, faeces specimen
  • Specimen Container: Viral swab (pink topped), faeces specimen in sterile universal container
  • Specimen Volume: 1.2g (spoonful) for faeces specimen
  • Specimen Requirements: Refrigerate if delivery to the lab is delayed
  • Other details: N/A
  • Frequency of analysis/ assay:Referred to external test site
  • Turnaround Time: 7-10 days
  • Phone alert limits:N/A
  • Preanalytical stability at room temp:N/A

Epanutin (Phenytoin)

 

  • Test Name: Epanutin (Phenytoin)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements:
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: 2 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Epilim (Valproic acid/sodium valporate)

 

  • Test Name: Epilim (Valproic acid/sodium valporate)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements:
  • Other details: Serum concentrations are no better a guide to clinical response than is the dose, therefore routine monitoring of valporate concentrations is not recommended. The measurement in psychiatric patients may be useful when compliance is an issue.
  • Frequency of analysis/ assay:
  • Turnaround Time: 2 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Erythrocyte Sedimentation Rate (ESR)

 

  • Test Name: Erythrocyte Sedimentation Rate (ESR)
  • Laboratory: Haematology
  • Specimen Type: EDTA
  • Specimen Container: Purple
  • Specimen Volume: 2.5ml
  • Specimen Requirements: Can be performed on same sample as FBC
  • Other details: When both an ESR and CRP are requested the CRP only is analysed; unless it is stated on the lab request form that the ESR is required for a particular clinical reason.
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: Routine - 2 working day
  • Phone alert limits:
  • Preanalytical stability at room temp: Samples should ideally be processed within 4 hours of collection. Please send to the laboratory ASAP.

 

Erythropoietin

 

  • Test Name: Erythropoietin
  • Laboratory: Haematology referral test
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4ml
  • Specimen Requirements: 
  • Other details: Sample to be taken in the morning.
  • Frequency of analysis/ assay: As required
  • Turnaround Time: 10 days
  • Phone alert limits: N/A
  • Preanalytical stability: 7 days at 2 - 8°C

 

Extractable nuclear antigens

 

  • Test Name: Extractable nuclear antigens
  • Laboratory: Autoantibody Lab.
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4 mls
  • Specimen Requirements: Refrigerate if delivery to the lab is delayed
  • Other details: ENA profile test (including SSA, SSB, Sm, RNP, Jo-1, Scl-70) is requested on all positive ENA screens
  • Frequency of analysis/ assay: Screening test carried out weekly
  • Turnaround Time: 10 days
  • Phone alert limits: N/A
  • Preanalytical stability at room temp: N/A

 

Eye infections (bacterial)

 

  • Test Name: Eye infections
  • Laboratory: Microbiology
  • Specimen Type: Swabs - Conjunctival / Eye, Corneal scraping, Contact lens / fluid
  • Specimen Container: Amies swab, sterile universal container.
  • Specimen Volume: At least 1ml of fluid
  • Specimen Requirements: Refrigerate if delay in delivery to the laboratory. Corneal Scraping innoculated directly onto culture plates/ slides and sent to laboratory
  • Other details: N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: Microscopy same day 2-4 days for culture
  • Phone alert limits:Beta Haemolytic Streptococcus Group A isolates, Beta Haemolytic Streptococcus Group B isolates from maternity, post natal and neonatal patients, new MRSA and VRE isolates from inpatients are all phoned to the requesting clinician
  • Preanalytical stability at room temp: N/A

 

Eye infections (viral)

  • Test Name: Eye infections
  • Laboratory: Serology External
  • Specimen Type: Swabs - Viral swab (pink topped)- Conjunctival / Eye, Corneal scraping, Contact lens / fluid
  • Specimen Container: Pink topped viral swab, sterile universal container.
  • Specimen Volume: At least 1ml of fluid
  • Specimen Requirements: Refrigerate if delay in delivery to the laboratory. 
  • Other details: N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: 7-10 days
  • Phone alert limits: Positive findings are phoned to the requesting clinician
  • Preanalytical stability at room temp: N/A

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